Dynamic light-scattering studies of internal motions of linear lambda-phage DNA in A- and B-forms

Mariya A. Ivanova; Alexandr V. Arutyunian; Valentin A. Noskin

doi:10.1117/12.148369

21 July 1993 Dynamic light-scattering studies of internal motions of linear λ-phage DNA in A- and B-forms

Mariya A. Ivanova, Alexandr V. Arutyunian, Valentin A. Noskin

Proceedings Volume 1884, Static and Dynamic Light Scattering in Medicine and Biology; (1993) https://doi.org/10.1117/12.148369
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Conformational changes of (lambda) -phage DNA macromolecules by dynamic light scattering (DLS) method were investigated. It was shown that the persistence length of B-form DNA could reduce to small value in some conditions, and could achieve approximately equals 12 nm. When studying the internal motions in the linear form of (lambda) -DNA, there was observed the deviation of the experimental angular dependence of the first cumulant (Gamma) from theoretical predictions for the (Gamma) in Rouse-Zimm model including hydrodynamic interactions. This deviation appears in region qR_g >> 1. The first cumulant deviation to the higher frequencies is observed only for B-form DNA. Experimental behavior of the first cumulant (Gamma) for the A-form DNA coincides with the theoretical one in the Rouse-Zimm model.

Citation Download Citation

Mariya A. Ivanova, Alexandr V. Arutyunian, and Valentin A. Noskin "Dynamic light-scattering studies of internal motions of linear λ-phage DNA in A- and B-forms", Proc. SPIE 1884, Static and Dynamic Light Scattering in Medicine and Biology, (21 July 1993); https://doi.org/10.1117/12.148369

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