Calculating the reduced scattering coefficient of turbid media from a single optical reflectance signal

Maureen Johns; Hanli Liu

doi:10.1117/12.477937

22 July 2003 Calculating the reduced scattering coefficient of turbid media from a single optical reflectance signal

Maureen Johns, Hanli Liu

Proceedings Volume 4958, Advanced Biomedical and Clinical Diagnostic Systems; (2003) https://doi.org/10.1117/12.477937
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

When light interacts with tissue, it can be absorbed, scattered or reflected. Such quantitative information can be used to characterize the optical properties of tissue, differentiate tissue types in vivo, and identify normal versus diseased tissue. The purpose of this research is to develop an algorithm that determines the reduced scattering coefficient (μ_s’) of tissues from a single optical reflectance spectrum with a small source-detector separation. The basic relationship between μ_s’ and optical reflectance was developed using Monte Carlo simulations. This produced an analytical equation containing μ_s’ as a function of reflectance. To experimentally validate this relationship, a 1.3-mm diameter fiber optic probe containing two 400-micron diameter fibers was used to deliver light to and collect light from Intralipid solutions of various concentrations. Simultaneous measurements from optical reflectance and an ISS oximeter were performed to validate the calculated μ_s’ values determined by the reflectance measurement against the 'gold standard’ ISS readings. The calculated μ_s’ values deviate from the expected values by approximately ∓ 5% with Intralipid concentrations between 0.5 − 2.5%. The scattering properties within this concentration range are similar to those of in vivo tissues. Additional calculations are performed to determine the scattering properties of rat brain tissues and to discuss accuracy of the algorithm for measured samples with a broad range of the absorption coefficient (μ_a).

Citation Download Citation

Maureen Johns and Hanli Liu "Calculating the reduced scattering coefficient of turbid media from a single optical reflectance signal", Proc. SPIE 4958, Advanced Biomedical and Clinical Diagnostic Systems, (22 July 2003); https://doi.org/10.1117/12.477937

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