High-frequency ultrasonic imaging of thickly sliced specimens

Chiaki Miyasaka; Bernhard R. Tittmann; Premindra Anthony N. Chandraratna

doi:10.1117/12.483984

1 August 2003 High-frequency ultrasonic imaging of thickly sliced specimens

Chiaki Miyasaka, Bernhard R. Tittmann, Premindra Anthony N. Chandraratna

Proceedings Volume 5047, Smart Nondestructive Evaluation and Health Monitoring of Structural and Biological Systems II; (2003) https://doi.org/10.1117/12.483984
Event: NDE for Health Monitoring and Diagnostics, 2003, San Diego, California, United States

Abstract

It has been reported that a mechanical scanning reflection acoustic microscope (hereinafter called simply "SAM"), using high frequency ultrasonic tone-burst waves, can form a horizontal cross-sectional image (i.e., c-scan image) showing a highly resolved cellular structure of biological tissue. However, the tissue prepared for the SAM has been mostly a thinly sectioned specimen. In this study, the SAM images of specimens thickly sectioned from the tissue were analyzed. Optical and scanning acoustic microscopies were used to evaluate tissues of human small intestine and esophagus. For preparing thin specimens, the tissue was embedded in paraffin, and substantially sectioned at 5-10μm by the microtome. For optical microscopy, the tissue was stained with hematoxylin and eosin, and affixed onto glass substrates. For scanning acoustic microscopy, two types of specimens were prepared: thinly sectioned specimens affixed on the glass substrate, wherein the specimens were deparaffinized in xylene, but not stained, and thickely sectioned specimens. Images of the thick specimens obtained with frequency at 200 MHz revealed cellular structures. The morphology was very similar to that seen in the thinly sectioned specimens with optical and scanning acoustic microscopy. In addition, scanning electron microscopy was used to compare the images of biological tissue. An acoustic lens with frequency at 200 MHz permitted the imaging of surface and/or subsurface of microstructures in the thick sections of small intestine and esophagus.

Citation Download Citation

Chiaki Miyasaka, Bernhard R. Tittmann, and Premindra Anthony N. Chandraratna "High-frequency ultrasonic imaging of thickly sliced specimens", Proc. SPIE 5047, Smart Nondestructive Evaluation and Health Monitoring of Structural and Biological Systems II, (1 August 2003); https://doi.org/10.1117/12.483984

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available