A novel method for accurate patterning and positioning of biological cells

Gaoshan Jing; Joseph P. Labukas; Aziz Iqbal; Susan Fueshko Perry; Gregory S. Ferguson; Svetlana Tatic-Lucic

doi:10.1117/12.722012

22 May 2007 A novel method for accurate patterning and positioning of biological cells

Gaoshan Jing, Joseph P. Labukas, Aziz Iqbal, Susan Fueshko Perry, Gregory S. Ferguson, Svetlana Tatic-Lucic

Proceedings Volume 6592, Bioengineered and Bioinspired Systems III; 65920Y (2007) https://doi.org/10.1117/12.722012
Event: Microtechnologies for the New Millennium, 2007, Maspalomas, Gran Canaria, Spain

Abstract

The ability to anchor cells in predefined patterns on a surface has become very important for the development of cell-based sensors, tissue-engineering applications, and the understanding of basic cell functions. Currently, the most widely used technique to generate micrometer or sub-micrometer-sized patterns for various biological applications is microcontact printing (&mgr;CP). However, the fidelity of the final pattern may be compromised by deformation of the PDMS stamps used during printing. A novel technique for accurately patterning and positioning biological cells is presented, which can overcome this obstacle. We have fabricated a chip on a silicon wafer using standard photolithographic and deposition processes consisting of gold patterns on top of PECVD silicon dioxide. A hydrophobic self-assembled monolayer (SAM) derived from 1-hexadecanethiol (HDT) was coated on the gold surface to prevent cell growth, and a hydrophilic SAM derived from (3-trimethoxysilyl propyl)-diethylenetriamine (DETA) was coated on the exposed PECVD silicon dioxide surface to promote cell growth. Immortalized mouse hypothalamic neurons (GT1-7) were cultured in vitro on the chip, and patterned cells were fluorescently stained and visualized by fluorescence microscopy. By our method, hydrophobic and hydrophilic regions can be reliably generated and easily visualized under a microscope prior to cell culturing. Cell growth was precisely controlled and limited to specific areas. The achieved resolution was 2 microns, and it could be improved with high resolution photolithographic methods.

Citation Download Citation

Gaoshan Jing, Joseph P. Labukas, Aziz Iqbal, Susan Fueshko Perry, Gregory S. Ferguson, and Svetlana Tatic-Lucic "A novel method for accurate patterning and positioning of biological cells", Proc. SPIE 6592, Bioengineered and Bioinspired Systems III, 65920Y (22 May 2007); https://doi.org/10.1117/12.722012

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