Refractive index measurement in human tissue samples

Vesselina Tsenova; Elena V. Stoykova

doi:10.1117/12.519584

4 November 2003 Refractive index measurement in human tissue samples

Vesselina Tsenova, Elena V. Stoykova

Proceedings Volume 5226, 12th International School on Quantum Electronics: Laser Physics and Applications; (2003) https://doi.org/10.1117/12.519584
Event: 12th International School on Quantum Electronics Laser Physics and Applications, 2002, Varna, Bulgaria

Abstract

The aim of this study was to apply a laser refractometer based on a total internal reflection for measurement of refractive indices of thin tissue samples. The tissue slices with thickness from 5 to 15 μm and cross-sectional area of about 1 - 1.5 cm were cut with a freezing microtome and mounted on microscope glass slides. The samples from a liver, kidney, myocardium and skin were taken post mortem from 3 male patients (50, 52 and 75 years old) with some pathological tissue changes as well as from 3 dead fetuses 24-48 hours after the death. To indicate the morphological changes, the samples were stained with hemolown-eosin (HE) and studied microscopically. Using a two-beam UV-Vis-NIR-spectrophotometer Cary-5E (Variant), on-axis transmittance of the samples was measured over the 350 - 2000 nm range and the spectra of the extinction coefficient, in cm^-1, were calculated. The measured refractive indices correspond to dehydrated tissue samples.

Citation Download Citation

Vesselina Tsenova and Elena V. Stoykova "Refractive index measurement in human tissue samples", Proc. SPIE 5226, 12th International School on Quantum Electronics: Laser Physics and Applications, (4 November 2003); https://doi.org/10.1117/12.519584

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