Studies on activated cytostatic fluorouracil as photosensitizer: to use in eye tumor treatment

Mihail-Lucian Pascu; Benone D. Carstocea; Mihail Brezeanu; Letitia Voicu; Angela Staicu; Doina M. Gazdaru; Ruxandra A. Pascu

doi:10.1117/12.584388

10 September 2004 Studies on activated cytostatic fluorouracil as photosensitizer: to use in eye tumor treatment

Mihail-Lucian Pascu, Benone D. Carstocea, Mihail Brezeanu, Letitia Voicu, Angela Staicu, Doina M. Gazdaru, Ruxandra A. Pascu

Author Affiliations +

Proceedings Volume 5610, Laser Florence 2003: A Window on the Laser Medicine World; (2004) https://doi.org/10.1117/12.584388
Event: Laser Florence 2003: A Window on the Laser Medicine World, 2003, Florence, Italy

Abstract

Hydroxypyrimidine 5-fluorouracil (5-FU) belongs to the cytostatics group known as antimetabolites. The effect of UV irradiation on 5-FU was investigated by absorption and fluorescence spectroscopy. The study of the photosensitizer properties of 5-FU was made since their effects could be enhanced by exposure to UV radiation at different doses. Solutions 2.5x10^-4M in natural saline water (0.8% NaCl), irradiated with optical beams emitted by N₂ laser and UV Hg classic lamp, were used. The 5-FU was chosen due to its strong absorption along a large spectral range which makes possible the fluorescence excitation in UV. The absorption spectra exhibit bands between 250 - 450 nm. The emission fluorescence was measured in the 400-550 nm spectral range, with λ_ex=320 and 350 nm for samples irradiated with Hg lamp and with λ_ex=360 nm for samples irradiated with N₂ laser. The excitation fluorescence was measured in the spectral range 200-400 nm, with λ_em=440 nm for samples irradiated with N₂ laser. The spectra reveal a fluorescence enhancement with the exposure time, with a maximum at 3 min due to the transformation of 5-FU molecule into a fluorescent tautomeric form. The destruction more rapid than usual of the neovascularisation was observed for conjunctive of rabbit eyes, when they are impregnated with 5-FU solution and exposed to incoherent UV and visible light.

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Mihail-Lucian Pascu, Benone D. Carstocea, Mihail Brezeanu, Letitia Voicu, Angela Staicu, Doina M. Gazdaru, and Ruxandra A. Pascu "Studies on activated cytostatic fluorouracil as photosensitizer: to use in eye tumor treatment", Proc. SPIE 5610, Laser Florence 2003: A Window on the Laser Medicine World, (10 September 2004); https://doi.org/10.1117/12.584388

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KEYWORDS

Luminescence

Ultraviolet radiation

Absorption

Lamps

Mercury

National Ignition Facility

Eye

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