Assessment of tumor angiogenesis using fluorescence contrast agents

Yu Chen; Qian Liu; Ping Huang; Shay Hyman; Xavier Intes; William Lee; Britton Chance

doi:10.1117/12.546210

8 December 2003 Assessment of tumor angiogenesis using fluorescence contrast agents

Yu Chen, Qian Liu, Ping Huang, Shay Hyman, Xavier Intes, William Lee, Britton Chance

Proceedings Volume 5254, Third International Conference on Photonics and Imaging in Biology and Medicine; (2003) https://doi.org/10.1117/12.546210
Event: Third International Conference on Photonics and Imaging in Biology and Medicine, 2003, Wuhan, China

Abstract

Angiogenesis is an important factor for further tumor growth and thus could be an attractive therapeutic target. Optical imaging can provide a non-invasive way to measure the permeability of tumor blood vessels and assess the tumor vasculature. We have developed a dual-channel near-infrared fluorescence system for simultaneous measurement of the pharmacokinetics of tumorous and normal tissues with exogenous contrast agents. This frequency-domain system consists of the light source (780 nm laser diode), fiber optics, interference filter (830 nm) and the detector (PMT). The fluorescent contrast agent used in this study is Indocyanine Green (ICG), and the normal dosage is 100 μl at a concentration of 5 μM. In vivo animal study is performed on the K1735 melanoma-bearing mouse. The fluorescence signals both tumorous and normal tissues after the bolus injection of ICG through the tail vein are continuously recorded as a function of time. The data is fitted by a double-exponential model to reveal the wash-in and wash-out parameters of different tissues. We observed an elongated wash-out from the tumor compared with normal tissue (leg). The effect of radiation therapy on the tumor vasculature is also discussed.

Citation Download Citation

Yu Chen, Qian Liu, Ping Huang, Shay Hyman, Xavier Intes, William Lee, and Britton Chance "Assessment of tumor angiogenesis using fluorescence contrast agents", Proc. SPIE 5254, Third International Conference on Photonics and Imaging in Biology and Medicine, (8 December 2003); https://doi.org/10.1117/12.546210

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