In vivo near-infrared spectroscopy of rat skin tissue with varying blood glucose levels

Jonathon T. Olesberg; Lingzhi Liu; Valerie Van Zee; Mark A. Arnold

doi:10.1117/12.529526

18 June 2004 In vivo near-infrared spectroscopy of rat skin tissue with varying blood glucose levels

Jonathon T. Olesberg, Lingzhi Liu, Valerie Van Zee, Mark A. Arnold

Proceedings Volume 5325, Optical Diagnostics and Sensing IV; (2004) https://doi.org/10.1117/12.529526
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

We have performed in vivo measurements of near-infrared rat skin absorption in the 4000-5000 cm^-1 spectral range (2.0-2.5 μm wavelength) during a glucose clamp experiment. The goal of this work is to identify the presence of glucose-specific spectral information in order to evaluate the requirements for a noninvasive transcutaneous glucose instrument. Skin spectra are collected using an FTIR spectrometer coupled with a fiber-optic interface. In the experiment, an animal is allowed to stabilize at a euglycemic level for three hours while blood glucose values are monitored using samples taken from an arterial catheter. The blood glucose level is then increased above 30 mM by venous infusion of glucose and held for two hours, after which it is allowed to return to normal. Spectra are recorded continuously during the procedure and are analyzed to identify changes due to the glucose variations. Because the change in absorbance due to an increase in glucose concentration is small compared to changes due to other variations (e.g., the thickness of the skin sample), a simple subtraction of absorbance spectra from the hyperglycemic and euglycemic phases is not instructive. Instead, a set of principal components is determined from the euglycemic period where the glucose concentration is constant. We then examine the change in absorbance during the hyperglycemic period that is orthogonal to these principal components. We find that there are significant similarities between these orthogonal variations and the net analyte signal of glucose, which suggests that glucose spectral information is present.

Citation Download Citation

Jonathon T. Olesberg, Lingzhi Liu, Valerie Van Zee, and Mark A. Arnold "In vivo near-infrared spectroscopy of rat skin tissue with varying blood glucose levels", Proc. SPIE 5325, Optical Diagnostics and Sensing IV, (18 June 2004); https://doi.org/10.1117/12.529526

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available