Observation of microarray DNA hybridization using surface plasmon resonance phase-shift interferometry

Shean-Jen Chen; C.-Y. Tsou; Y.-K. Chen; Y.-T. Su

doi:10.1117/12.530568

16 June 2004 Observation of microarray DNA hybridization using surface plasmon resonance phase-shift interferometry

Shean-Jen Chen, C.-Y. Tsou, Y.-K. Chen, Y.-T. Su

Proceedings Volume 5327, Plasmonics in Biology and Medicine; (2004) https://doi.org/10.1117/12.530568
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Surface plasmon resonance phase-shift interferometry (SPR-PSI) is a novel technique which combines SPR and modified Mach-Zehnder phase-shifting interferometry to measure the spatial phase variation caused by biomolecular interactions upon a sensing chip. The SPR-PSI imaging system offers high resolution and high-throughout screening capabilities for microarray DNA hybridization without the need for additional labeling, and provides valuable real-time quantitative information. Current SPR-PSI imaging systems measure the spatial phase variation caused by tiny biomolecular changes on the sensing interface by means of a five-step phase reconstruction algorithm and a novel multichannel least mean squares (MLMS) phase unwrapping algorithm. The SPR-PSI imaging system has an enhanced detection limit of 2.5 × 10^-7refraction index change, a long-term phase stability of π/100 in 30 minutes, and a spatial phase resolution of π/500 with a lateral resolution of 10μm. This study successfully demonstrates the kinetic and label-free observation of 5-mer DNA microarray hybridization.

Citation Download Citation

Shean-Jen Chen, C.-Y. Tsou, Y.-K. Chen, and Y.-T. Su "Observation of microarray DNA hybridization using surface plasmon resonance phase-shift interferometry", Proc. SPIE 5327, Plasmonics in Biology and Medicine, (16 June 2004); https://doi.org/10.1117/12.530568

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available