Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Investigation of photoacoustic spectroscopy for biomolecular detection

[+] Author Affiliations
Saher M. Maswadi, Randolph D. Glickman

Univ. of Texas Health Science Ctr. at San Antonio

Norman Barsalou, Rowe W. Elliott

Naval Health Research Ctr.

Proc. SPIE 6138, Ophthalmic Technologies XVI, 61380V (March 07, 2006); doi:10.1117/12.674408
Text Size: A A A
From Conference Volume 6138

  • Ophthalmic Technologies XVI
  • Fabrice Manns; Per G. Söderberg; Arthur Ho
  • San Jose, CA | January 21, 2006

abstract

We are developing a non- or minimally-invasive method for detecting and measuring specific drugs and biomolecules in vivo using photoacoustic spectroscopy (PAS). This pilot study investigated the feasibility of detecting the concentration of certain drugs in the vitreous or aqueous of the eye. As a prototype for using PAS for molecular detection in vivo, the technique was applied to the detection in a surrogate eye, of drugs with known optical spectrum such as Trypan Blue, Rose Bengal, and Amphotericin B (AB), at concentrations as low as 1 μg/ml. Chopped CW, or short pulse, Q-switch lasers, were used as pumping sources to generate ultrasonic photoacoustic signals in an ocular phantom containing the drug solutions. In addition to an ultrasonic hydrophone, the photothermal deflection technique (PhDT), a non-contact optical method with high sensitivity and fast response, were used to record the photoacoustic signals. The data from both detectors were compared over a range of drug concentrations. The photoacoustic signal generated from the retina was used as a reference, to measure the attenuation of light through drug solutions of different concentrations in the ocular phantom. The results indicated that photoacoustic spectroscopy is feasible in ocular phantoms incorporating ex vivo ocular tissue. The signals recorded using PAS were to be found to be linearly dependent on drug concentration, as predicted by theory. The photoacoustic method was found to be sensitive to drug concentrations as low as 1 μg/ml, a clinically relevant concentration for many drugs. Future work will be directed at adapting this method for in vivo measurement, and enhancing its sensitivity by using a tunable laser as the pump source.

© (2006) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Saher M. Maswadi ; Randolph D. Glickman ; Norman Barsalou and Rowe W. Elliott
"Investigation of photoacoustic spectroscopy for biomolecular detection", Proc. SPIE 6138, Ophthalmic Technologies XVI, 61380V (March 07, 2006); doi:10.1117/12.674408; http://dx.doi.org/10.1117/12.674408


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.