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Proceedings Article

Infrared imaging of normal and diseased cervical tissue sections

[+] Author Affiliations
Bayden R. Wood, Keith R. Bambery, Don McNaughton

Monash Univ. (Australia)

Lisa M. Miller

Brookhaven National Lab. (USA)

Michael Quinn

Royal Women's Hospital (Australia)

Luis Chiriboga

Bellevue Hosp./NYU Medical Ctr. (USA)

Max Diem

CUNY/Hunter College (USA)

Proc. SPIE 5651, Biomedical Applications of Micro- and Nanoengineering II, 78 (March 09, 2005); doi:10.1117/12.582294
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From Conference Volume 5651

  • Biomedical Applications of Micro- and Nanoengineering II
  • Dan V. Nicolau
  • Sydney, Australia | December 12, 2004

abstract

Synchrotron FTIR maps, focal plane array and linear array images recorded of 4 μm cervical biopsy sections from the surface epithelium and glandular endometrium are compared in terms of spatial resolution and applicability to the clinical environment. Synchrotron FTIR maps using a 10 μm aperture appear to provide a better spatial resolution capable of discerning single nuclei in the tissue matrix. Unsupervised hierarchical cluster analysis performed on the synchrotron, focal plane array and linear array data in the 1700-1400 cm-1 region show very similar clusters and mean-extracted spectra, demonstrating the robustness of FTIR microscopy and UHCA in the analysis of tissue sections. Maps recorded with the focal plane array using a conventional globar source take one-fortieth of the time but the spatial resolution precludes true single cell analysis in the tissue matrix. The high spatial resolution achieved with the synchrotron shows potential as a gold standard for FTIR diagnosis of cervical samples.

© (2005) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Bayden R. Wood ; Keith R. Bambery ; Lisa M. Miller ; Michael Quinn ; Luis Chiriboga, et al.
"Infrared imaging of normal and diseased cervical tissue sections", Proc. SPIE 5651, Biomedical Applications of Micro- and Nanoengineering II, 78 (March 09, 2005); doi:10.1117/12.582294; http://dx.doi.org/10.1117/12.582294


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