Full-field OCT: ex vivo and in vivo biological imaging applications

Katharine Grieve; Arnaud Dubois; Gael Moneron; Elvire Guyot; Albert Claude Boccara

doi:10.1117/12.589651

13 April 2005 Full-field OCT: ex vivo and in vivo biological imaging applications

Katharine Grieve, Arnaud Dubois, Gael Moneron, Elvire Guyot, Albert Claude Boccara

Proceedings Volume 5690, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine IX; (2005) https://doi.org/10.1117/12.589651
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

We present results of studies in embryology and ophthalmology performed using our ultrahigh-resolution full-field OCT system. We also discuss recent developments to our ultrashort acquisition time full-field optical coherence tomography system designed to allow in vivo biological imaging. Preliminary results of high-speed imaging in biological samples are presented. The core of the experimental setup is the Linnik interferometer, illuminated by a white light source. En face tomographic images are obtained in real-time without scanning by computing the difference of two phase-opposed interferometric images recorded by high-resolution CCD cameras. An isotropic spatial resolution of ~1 μm is achieved thanks to the short source coherence length and the use of high numerical aperture microscope objectives. A detection sensitivity of ~90 dB is obtained by means of image averaging and pixel binning. In ophthalmology, reconstructed xz images from rat ocular tissue are presented, where cellular-level structures in the retina are revealed, demonstrating the unprecedented resolution of our instrument. Three-dimensional reconstructions of the mouse embryo allowing the study of the establishment of the anterior-posterior axis are shown. Finally we present the first results of embryonic imaging using the new rapid acquisition full-field OCT system, which offers an acquisition time of 10 μs per frame.

Citation Download Citation

Katharine Grieve, Arnaud Dubois, Gael Moneron, Elvire Guyot, and Albert Claude Boccara "Full-field OCT: ex vivo and in vivo biological imaging applications", Proc. SPIE 5690, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine IX, (13 April 2005); https://doi.org/10.1117/12.589651

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