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Proceedings Article

Ultrasensitive time-resolved nanoliter volume fluorometry based on UV LEDs and a channel photomultiplier tube

[+] Author Affiliations
Dayong Jin, Russell Connally, James Piper

Macquarie Univ. (Australia)

Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, 237 (April 11, 2005); doi:10.1117/12.590127
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From Conference Volume 5699

  • Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III
  • Dan V. Nicolau; Joerg Enderlein; Robert C. Leif; Daniel L. Farkas; Ramesh Raghavachari
  • San Jose, CA | January 22, 2005

abstract

A capillary fluorometer was constructed using a 2 mW, 365 nm ultraviolet (UV) light emitting diode (LED) as the excitation source and a new-generation high-gain (3×108) channel photomultiplier tube. The use of a LED permitted rapid pulsing of the excitation source so that the instrument could be employed for time-resolved fluorescence (TRF) applications. A detection limit of ~2×108 molecules of BHHT (4,4"-bis (1",1",1",2",2",3",3"-heptafluoro-4",6"-hexanedion-6"yl)-o-tephenyl)-Eu (III) were resolved within a 1.25 nanoliter volume at a S/N ratio of 3:1. Ultimate sensitivity of the system was compromised due to visible luminescence emitted by the UV LED, centred around 550 nm extending to > 700 nm and 2nd-order exponentially decaying with lifetimes of 40 μs and 490 μs.

© (2005) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Dayong Jin ; Russell Connally and James Piper
"Ultrasensitive time-resolved nanoliter volume fluorometry based on UV LEDs and a channel photomultiplier tube", Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, 237 (April 11, 2005); doi:10.1117/12.590127; http://dx.doi.org/10.1117/12.590127


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