Development and preliminary results of an in vivo Raman probe for early lung cancer detection

Michael A. Short; Stephen Lam; Annette McWilliams; Jianhua Zhao; Harvey Lui; Haishan Zeng

doi:10.1117/12.764034

8 February 2008 Development and preliminary results of an in vivo Raman probe for early lung cancer detection

Michael A. Short, Stephen Lam, Annette McWilliams, Jianhua Zhao, Harvey Lui, Haishan Zeng

Proceedings Volume 6853, Biomedical Optical Spectroscopy; 68530J (2008) https://doi.org/10.1117/12.764034
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Our previous results from Raman spectroscopy studies on ex vivo lung tissue showed the technique had great potential to differentiate between samples with different pathologies. In this work, a fast dispersive-type near-infrared (NIR) Raman spectroscopy system was developed to collect real-time, noninvasive, in vivo human lung spectra. The 785 nm excitation, and the collection of tissue emission were accomplished by using a reusable fiber optic catheter which passed down the instrument channel of a bronchoscope. Filters in two stages blocked laser emission other than 785 nm from reaching the tissue surface, and reduced fiber fluorescence and elastically scattered excitation light from being passed to the spectrometer. The spectrometer itself consisted of one of two holographic gratings with usable frequency ranges of: 700 to 2000 cm^-1 and 1500 to 3400 cm^-1. The dispersed light was detected by a cooled CCD array consisting of 400 by 1340 pixels. To increase the resolution of the system, while maximizing the throughput, a second fiber bundle, consisting of 54×100 μm diameter fibers connected the catheter to the spectrometer. The fibers in this second bundle were spread out to form a parabolic arc which replaced the conventional entrance slit. This geometry corrected for image aberrations, permitting complete CCD vertical binning, thereby yielding up to a 20-fold improvement in signal-to-noise ratio. The estimated spectral resolution of the system was 9 cm^-1 for both gratings. So far we have measured spectra from 20 patients and have seen clear differences between spectra from tumor and normal tissue.

Citation Download Citation

Michael A. Short, Stephen Lam, Annette McWilliams, Jianhua Zhao, Harvey Lui, and Haishan Zeng "Development and preliminary results of an in vivo Raman probe for early lung cancer detection", Proc. SPIE 6853, Biomedical Optical Spectroscopy, 68530J (8 February 2008); https://doi.org/10.1117/12.764034

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