Study of lipid rich compositions in the intimal wall of aorta by Raman spectroscopy

C.-H. Liu; V. Sriramoju; W. B. Wang; M. Frankfurt; N. D. Zadeh; R. R. Alfano V.D.M.

doi:10.1117/12.767333

8 February 2008 Study of lipid rich compositions in the intimal wall of aorta by Raman spectroscopy

C.-H. Liu, V. Sriramoju, W. B. Wang, M. Frankfurt, N. D. Zadeh, R. R. Alfano V.D.M.

Proceedings Volume 6853, Biomedical Optical Spectroscopy; 68531E (2008) https://doi.org/10.1117/12.767333
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

We report an ex-vivo study on Raman spectra of adipose tissue covered by layers of aortic intimal wall tissue with different thicknesses. The Raman vibration modes of 1435cm^-1, 2850cm^-1 and 2892cm^-1 were investigated for the first time on fresh porcine aortic adipose tissue with 633 nm laser excitation. The adipose tissue was taken from adventitial fat grown on aorta walls. The frozen sections of porcine aorta wall tissue with a thickness of 25μ - 50μ were cut from intimal surface. The samples were prepared by placing the variable numbers of the aorta intimal wall tissue layers on the top of adipose tissue. The Raman spectra of adipose tissue were investigated. The changes of intensities of the Raman modes versus thickness of the aorta intimal wall tissue layers were measured. The total thickness of the aorta intimal wall tissue layers was varied in the range of 50μ - 1800μ. The main characteristic Raman vibration modes of adipose tissue were found at 1435cm^-1, 2850cm^-1 and 2892cm^-1. Among them, the intensities of the modes of 2850 cm^-1 and 2892 cm^-1 are about four-times stronger than that of the 1435cm^-1 mode. The study on Raman vibration modes of 1435cm^-1, 2800 cm^-1 and 2950 cm^-1 may be useful for developing a simple, inexpensive and accurate optical technique for monitoring the degree of vulnerability of the aorta intimal surface due to atherogenesis. These three Raman modes can be used as new molecular spectroscopic indicators to monitor in situ the development of fatty-streaks and lipid core in aorta walls, and determine the thickness change of aorta intimal wall at different stages of atherogenesis.

Citation Download Citation

C.-H. Liu, V. Sriramoju, W. B. Wang, M. Frankfurt, N. D. Zadeh, and R. R. Alfano V.D.M. "Study of lipid rich compositions in the intimal wall of aorta by Raman spectroscopy", Proc. SPIE 6853, Biomedical Optical Spectroscopy, 68531E (8 February 2008); https://doi.org/10.1117/12.767333

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