Rapid Raman microscopic imaging for potential histological screening

Joanne Hutchings; Catherine Kendall; Neil Shepherd; Hugh Barr; Brian Smith; Nicholas Stone

doi:10.1117/12.786440

8 February 2008 Rapid Raman microscopic imaging for potential histological screening

Joanne Hutchings, Catherine Kendall, Neil Shepherd, Hugh Barr, Brian Smith, Nicholas Stone

Proceedings Volume 6853, Biomedical Optical Spectroscopy; 685305 (2008) https://doi.org/10.1117/12.786440
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Raman spectroscopy has proved to be a highly sensitive tool for differentiating between normal, cancerous or pre-cancerous tissues. To date, histological application of Raman mapping has been limited due to lengthy mapping times. Streamline^TM Raman imaging is a novel mapping technique that has reduced total mapping times to a level that is becoming clinically practicable. Raman Streamline mapping was carried out on a 20μm frozen section of an oesophageal biopsy. A contiguous 7μm section was stained with haematoxylin and eosin (H&E) with histpathology analysed by a pathologist. The step size and acquisition times were varied and the resulting spectra, principal component score maps and loads were compared. The signal to noise for the raw spectra and a relative 'signal to noise' of the principal component loads were determined. The Streamline mapping technique was also compared to traditional point Raman mapping. The principal component loads were similar despite varying the acquisition time and number of spectra, with the fifth load used for comparison of the noise levels. Gross biochemical information was extracted showing good correlation with the H&E section even for short overall mapping times as low as 30-90 minutes for a biopsy ~2mm in diameter (0.5s acquisition time per 25.3μm Raman pixel). Streamline mapping was of the order of 3-7 times faster than traditional point mapping with the greatest improvement made for high resolution maps. Further optimization of the system is still possible which will reduce this mapping time further making implementation in a clinical environment a future possibility.

Citation Download Citation

Joanne Hutchings, Catherine Kendall, Neil Shepherd, Hugh Barr, Brian Smith, and Nicholas Stone "Rapid Raman microscopic imaging for potential histological screening", Proc. SPIE 6853, Biomedical Optical Spectroscopy, 685305 (8 February 2008); https://doi.org/10.1117/12.786440

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