Optimised 3D surface measurement of hydroxyapatite layers using adapted white light scanning interferometry

Emilia Pecheva; Paul Montgomery; Denis Montaner; Lilyana Pramatarova; Zenko Zanev

doi:10.1117/12.696011

15 September 2006 Optimised 3D surface measurement of hydroxyapatite layers using adapted white light scanning interferometry

Emilia Pecheva, Paul Montgomery, Denis Montaner, Lilyana Pramatarova, Zenko Zanev

Author Affiliations +

Proceedings Volume 6341, Speckle06: Speckles, From Grains to Flowers; 634137 (2006) https://doi.org/10.1117/12.696011
Event: Speckle06: Speckles, From Grains to Flowers, 2006, Nimes, France

Abstract

Biomineralization is intensively studied at present due to its importance in the formation of bones, teeth, cartilage, etc. Hydroxyapatite is one of the most common natural biomaterials and the primary structural component of bones and teeth. We have grown bio-like hydroxyapatite layers in-vitro on stainless steel, silicon and silica glass by using a biomimetic approach (immersion in a supersaturated aqueous solution resembling the ion composition of human blood plasma). Using classical techniques such as stylus profiling, AFM or SEM, it was found difficult, destructive or time-consuming to measure the topography, thickness and profile of the heterogeneous, thick and rough hydroxyapatite layers. White light scanning interferometry, on the other hand, has been found to be particularly useful for analyzing such bio-like layers, requiring no sample preparation and being rapid and non-destructive. The results have shown a typical layer thickness of up to 20 μm and a rms roughness of 4 μm. The hydroxyapatite presents nonetheless a challenge for this technique because of its semi-translucence, high roughness and the presence of cavities within its volume. This results in varying qualities of fringe pattern depending on the area, ranging from classical fringes on smooth surfaces, to complex speckle-like fringes on rough surfaces, to multiple fringe signals along the optical axis in the presence of buried layers. In certain configurations this can affect the measurement precision. In this paper we present the latest results for optimizing the measurement conditions in order to reduce such errors and to provide additional useful information concerning the layer.

Citation Download Citation

Emilia Pecheva, Paul Montgomery, Denis Montaner, Lilyana Pramatarova, and Zenko Zanev "Optimised 3D surface measurement of hydroxyapatite layers using adapted white light scanning interferometry", Proc. SPIE 6341, Speckle06: Speckles, From Grains to Flowers, 634137 (15 September 2006); https://doi.org/10.1117/12.696011

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