Light microscopy adapter for laser based microscopy techniques

Mojmír Šerý; Antonín Pochylý; Zedenk Lošťák; Petr Jákl; Pavel Zemánek

doi:10.1117/12.822358

20 November 2008 Light microscopy adapter for laser based microscopy techniques

Mojmír Šerý, Antonín Pochylý, Zedenk Lošťák, Petr Jákl, Pavel Zemánek

Proceedings Volume 7141, 16th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics; 71410D (2008) https://doi.org/10.1117/12.822358
Event: 16th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 2008, Polanica Zdroj, Poland

Abstract

Rapid development of micromanipulation and experimental techniques increases the need for easy-to-handle tools, which are suitable for routine operations. Our new concept represents a very suitable platform for incorporating such new experimental techniques into standard laboratory microscopy equipment. Implementing of microscopy methods such as optical trapping, cutting, fluorescence excitation, photopolymerization and most spectroscopic techniques is very expensive and time consuming. In most cases, microscope parts and body must be adapted to insert additional lasers, detectors etc. We overcome these difficulties by inserting source of radiation directly between microscope body and microscope objective. Our concept allows using arbitrary source of radiation (laser diodes, solid-state lasers, gas lasers). Laser diode source can be incorporated inside the adapter. Optical fiber is used to deliver other sources of radiation. Light microscopy adapter (LMA) can be easily mounted on most types of microscopes including special types as confocal microscope. We demonstrate ability of LMA for stable optical trapping of particle in the range of hundreds of nanometers to tens of micrometers, bleaching (FRAP, FLIP) and microdissection experiments. Mechanical and optical construction of LMA addresses spatial constrains of commercially available microscopes, mainly the fact that optical parts of microscopes are optimized for visible spectrum of light. Our solution preserves all microscope properties unaffected and it is possible modern microscopy techniques to many types of microscopes.

Citation Download Citation

Mojmír Šerý, Antonín Pochylý, Zedenk Lošťák, Petr Jákl, and Pavel Zemánek "Light microscopy adapter for laser based microscopy techniques", Proc. SPIE 7141, 16th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 71410D (20 November 2008); https://doi.org/10.1117/12.822358

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Microscopes

Microscopy

Semiconductor lasers

Objectives

Optical tweezers

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