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Proceedings Article

Angular domain fluorescent lifetime imaging in turbid media

[+] Author Affiliations
Fartash Vasefi

Simon Fraser Univ. (Canada) and Lawson Health Research Institute (Canada)

Eldon Ng, Jeffrey J. L. Carson

Lawson Health Research Institute (Canada) and Univ. of Western Ontario (Canada)

Bozena Kaminska, Glenn H. Chapman

Simon Fraser Univ. (Canada)

Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71830I (February 25, 2009); doi:10.1117/12.809458
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From Conference Volume 7183

  • Multiphoton Microscopy in the Biomedical Sciences IX
  • Ammasi Periasamy; Peter T. C. So
  • San Jose, CA | January 24, 2009

abstract

We describe a novel florescent lifetime imaging methodology applicable to fluorophores embedded in turbid media. The method exploits the collimation detection capabilities of an angular filter device to extract photons emitted by a fluorophore embedded at depth within the medium. A laser source is used to excite the fluorophore within the medium. Photons emitted by the fluorophore that are not scattered to a high degree pass through the angular filter array and are detected by the intensified CCD camera (200 ps minimum gate width). Scattered photons are rejected by the filter and do not pass through to the camera. We fabricated angular filter arrays using silicon bulk micromachining and found that an array of 80 μm square aperture micro-tunnels, 1.5 cm in length accepted photons with trajectories within 0.4° of the axes of the micro-tunnels. The small acceptance angle rejected most of the scattered light exiting the turbid medium.

© (2009) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Fartash Vasefi ; Eldon Ng ; Bozena Kaminska ; Glenn H. Chapman and Jeffrey J. L. Carson
"Angular domain fluorescent lifetime imaging in turbid media", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71830I (February 25, 2009); doi:10.1117/12.809458; http://dx.doi.org/10.1117/12.809458


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