Ultra-compact (palm-top size) low-cost maintenance-free (>3000 h) diode-pumped femtosecond (160 fs) solid state laser source for multiphoton microscopy

S. Yamazoe; M. Katou; T. Kasamatsu

doi:10.1117/12.808082

25 February 2009 Ultra-compact (palm-top size) low-cost maintenance-free (>3000 h) diode-pumped femtosecond (160 fs) solid state laser source for multiphoton microscopy

S. Yamazoe, M. Katou, T. Kasamatsu

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Proceedings Volume 7183, Multiphoton Microscopy in the Biomedical Sciences IX; 718334 (2009) https://doi.org/10.1117/12.808082
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

We propose and demonstrate a novel practical femtosecond laser source, which is to our knowledge, the smallest size and potentially low cost. The innovation is the simple linear cavity design utilizing soliton mode-locking induced by precise group velocity dispersion control. Average output power of 680 mW and pulse width of 162 fs were obtained at around 1045 nm from a diode-pumped Yb³⁺:KY(WO₄)₂ laser. The pulse repetition rate was 2.8 GHz, leading to a pulse peak power of 1.5 kW, which is sufficient for biomedical imaging. The laser module including the laser diode pump system has a footprint of 8×4 cm². Under automatic current control condition, stable operation of 3000-hour was observed with an average power fluctuation of less than ±10 %. Furthermore, under automatic power control condition, stable operation of 2000-hour was observed with an average power fluctuation of less than ±1 %. Using this laser module, we successfully obtained clear two-photon fluorescence images of muntjac skin fibroblast cells stained with a combination of fluorescent stains (Alexa Fluor 488 phalloidin and Alexa Flour 555 goat anti-mouse lgG).

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S. Yamazoe, M. Katou, and T. Kasamatsu "Ultra-compact (palm-top size) low-cost maintenance-free (>3000 h) diode-pumped femtosecond (160 fs) solid state laser source for multiphoton microscopy", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 718334 (25 February 2009); https://doi.org/10.1117/12.808082

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KEYWORDS

Mode locking

Femtosecond phenomena

Luminescence

Solitons

Automatic control

Dispersion

Semiconductor lasers

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