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The cornea contributes about 65% of the eye's ability to refract light. Thus, any fluctuation in corneal thickness can
cause noticeable changes in vision. The presence of glucose molecules induces a driving force for water to leave the
collagen fibrils in the cornea due to the concentration gradient created, thus changing its thickness. In this study, the
effect of various milli-molar glucose concentrations on corneal thickness was explored using Optical Coherence
Tomography. Whole rabbit eyes were placed in a specially designed dish while immersed in saline to ensure proper
hydration of the eye. The cornea was imaged for 10 minutes. In 30 minute increments, a higher concentration of
glucose was added, bringing the overall glucose concentration to 10, 15, 20, 25, and 30 mM. The thickness of the
cornea was measured every 2 minutes. Ultimately, an inverse relationship was observed, indicating that the increase
in glucose concentration yielded a decrease in the corneal thickness. From three separate experiments, the cornea
experienced 8 ± 1, 27 ± 1, 44 ± 3, 58 ± 3, and 64 ± 3 μm decrease in thickness from its starting value while exposed
to 10, 15, 20, 25, and 30 mM solutions of glucose, respectively. This relationship provides insight on the
physiological changes of the cornea as a result of different glucose concentrations. This could potentially be useful
in monitoring blood-glucose levels through the eye.
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