Wavelength-dependent backscattering measurements for quantitative real-time detection of apoptosis in living cells

Christine S. Mulvey; Carly A. Sherwood; Irving J. Bigio

doi:10.1117/12.808207

24 February 2009 Wavelength-dependent backscattering measurements for quantitative real-time detection of apoptosis in living cells

Christine S. Mulvey, Carly A. Sherwood, Irving J. Bigio

Author Affiliations +

Proceedings Volume 7187, Biomedical Applications of Light Scattering III; 71870M (2009) https://doi.org/10.1117/12.808207
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Our goal is to demonstrate the use of in vitro elastic scattering spectroscopy (ESS) to monitor changes in light-scattering properties of cells due to apoptotic micro-morphology changes. We developed an instrument capable of wavelength-resolved ESS measurements from cell cultures in the backward direction along with an algorithm to extract the size distribution of scatterers in the sample using Mie theory. CHO cells were cultured to confluence on plates and were rendered apoptotic with 2μM staurosporine. Backscattering measurements were performed on pairs of treated and control samples at times up to 6 hours post treatment. Initial results indicate that ESS is capable of discriminating between treated and control samples as early as 15 minutes post treatment. Extracted size distributions from treated and control samples show an increase in the number of small particles with a corresponding decrease in larger particles after treatment. This is consistent with expected morphological changes during apoptosis, and work continues to correlate these size distributions with underlying morphology.

Citation Download Citation

Christine S. Mulvey, Carly A. Sherwood, and Irving J. Bigio "Wavelength-dependent backscattering measurements for quantitative real-time detection of apoptosis in living cells", Proc. SPIE 7187, Biomedical Applications of Light Scattering III, 71870M (24 February 2009); https://doi.org/10.1117/12.808207

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Scattering

Cell death

Particles

Mie scattering

Backscatter

Light scattering

Optical spheres

Show All Keywords

Keywords/Phrases

Search In:

Publication Years