Using white-light spectroscopy for size determination of tissue phantoms

Elina A. Vitol; Timothy P. Kurzweg; Bahram Nabet

doi:10.1117/12.629728

13 October 2005 Using white-light spectroscopy for size determination of tissue phantoms

Elina A. Vitol, Timothy P. Kurzweg, Bahram Nabet

Proceedings Volume 5969, Photonic Applications in Biosensing and Imaging; 59691H (2005) https://doi.org/10.1117/12.629728
Event: Photonics North, 2005, Toronto, Canada

Abstract

Along with breast and cervical cancer, esophageal adenocarcinoma is one of the most common types of cancers. The characteristic features of pre-cancerous tissues are the increase in cell proliferation rate and cell nuclei enlargement, which both take place in the epithelium of human body surfaces. However, in the early stages of cancer these changes are very small and difficult to detect, even for expert pathologists. The aim of our research is to develop an optical probe for in vivo detection of nuclear size changes using white light scattering from cell nuclei. The probe will be employed through an endoscope and will be used for the medical examination of the esophagus. The proposed method of examination will be noninvasive, cheap, and specific, compared to a biopsy. Before the construction of this probe, we have developed theory to determine the nuclei size from the reflection data. In this first stage of our research, we compare experimental and theoretical scattered light intensities. Our theoretical model includes the values of scatterer size from which we can extract the nuclei size value. We first performed the study of polystyrene microspheres, acting as a tissue phantom. Spectral and angular distributions of scattered white light from tissue phantoms were studied. Experimental results show significant differences between the spectra of microspheres of different sizes and demonstrate almost linear relation between the number of spectral oscillations and the size of microspheres. Best results were achieved when the scattered light spectrum was collected at 30° to the normal of the sample surface. We present these research results in this paper. In ongoing work, normal and cancerous mammalian cell studies are being performed in order to determine cell nuclei size correlation with the size of microspheres through the light scattering spectrum observation.

Citation Download Citation

Elina A. Vitol, Timothy P. Kurzweg, and Bahram Nabet "Using white-light spectroscopy for size determination of tissue phantoms", Proc. SPIE 5969, Photonic Applications in Biosensing and Imaging, 59691H (13 October 2005); https://doi.org/10.1117/12.629728

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