Two-photon polymerization has emerged as a technology for rapid fabrication of three-dimensional micro-structures with nanoscale resolution. Commonly, a Ti:Sapphire femtosecond laser (operating at 780-800 nm wavelength) working at MHz pulse repetition rate is applied as an irradiation source to photomodify the resin. We present a system for pinpoint two-photon polymerization which utilizes second harmonic (515 nm) of amplified Yb:KGW femtosecond laser working at 312.5 kHz pulse repetition rate. Shorter irradiation wavelength enables one to focus laser beam to a smaller spot. High repetition rate and high average power capacitates rapid fabrication of three-dimensional structures over a large area. Results obtained prove the highest resolution of fabrication to be up to ~100 nm, and reproducible resolution 200 nm. Some micro-structures fabricated rapidly over millimeter area and revealing the specific problems arising at high speed fabrication over large lateral dimensions are presented in this report. Results obtained show the system and technologies applied to be well suitable for future routine 3D structuring over the large area and application of such structures in photonics, micro-optics, micromechanics, microelectronics and cell growth for tissue engineering.© (2009) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.