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Proceedings Article

Noninvasive diode laser activation of transient receptor potential proteins and nociceptors

[+] Author Affiliations
Nan Jiang, Brian Y. Cooper

Univ. of Florida

Michael I. Nemenov

Stanford Univ. and LasMed LLC

Proc. SPIE 6428, Mechanisms for Low-Light Therapy II, 642809 (February 21, 2007); doi:10.1117/12.699204
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From Conference Volume 6428

  • Mechanisms for Low-Light Therapy II
  • Michael R. Hamblin; Ronald W. Waynant; Juanita Anders
  • San Jose, CA | January 20, 2007

abstract

We investigated diode laser (980 nm) evoked activation of transient receptor potential proteins (TRPV1 and TRPV2). C and A-delta (Aδ) nociceptor families are primarily responsible for pain mediation in the peripheral nervous system. TRPV1 proteins have been associated with heat evoked pain in C fibers while Aδ fibers have been associated with TRPV2. Diode laser stimulation allows a margin of safety between non-invasive activation and damage 19, 22, 34. Laser pulses (20-50 ms, 0.1-10 W, 980 nm) were used to stimulate: A) in vitro: excised patches from HEK293 cells expressing TRPV1; B) in vitro : rat DRG nociceptors expressing either TRPV1 or TRPV2; and C) in vivo : C-fibers of the rat saphenous nerve (SN) trunk. Cell currents were recorded using standard patch clamp methods. The SN was also stimulated electrically with bipolar electrodes. Stimulation (20-50 ms) of HEK and DRG cells expressing TRPV1 was highly reproducible. Activation and peak currents were achieved at estimated peak temperatures of 55°C and 70°C. Threshold activation was also observed in DRG neurons expressing TRPV2. The conduction velocity for laser-activated saphenous nerve afferents was in the C fiber range (0.5-1 m/s). Electrically stimulated nerve contained stimulation artifacts and complex neural components with conduction velocities ranging from 0.3-30 m/s. Diode laser activation of TRPV1 protein is a reproducible and effective means to probe TRP activity in both in vivo and in vitro preparations

© (2007) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Nan Jiang ; Brian Y. Cooper and Michael I. Nemenov
"Noninvasive diode laser activation of transient receptor potential proteins and nociceptors", Proc. SPIE 6428, Mechanisms for Low-Light Therapy II, 642809 (February 21, 2007); doi:10.1117/12.699204; http://dx.doi.org/10.1117/12.699204


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