Changes in intrinsic optical signals during loss of tissue viability of brains in rats: effect of brain temperature

Satoko Kawauchi; Shunichi Sato; Hidetoshi Ooigawa; Hiroshi Nawashiro; Makoto Kikuchi

doi:10.1117/12.700209

26 February 2007 Changes in intrinsic optical signals during loss of tissue viability of brains in rats: effect of brain temperature

Satoko Kawauchi, Shunichi Sato, Hidetoshi Ooigawa, Hiroshi Nawashiro, Makoto Kikuchi

Author Affiliations +

Proceedings Volume 6434, Optical Tomography and Spectroscopy of Tissue VII; 64341O (2007) https://doi.org/10.1117/12.700209
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

To investigate the temperature dependence of intrinsic optical signals (IOSs) relating to brain tissue viability, we performed simultaneous measurement of light absorption due to the redox states of cytochrome c oxidase and light scattering, which reflects morphological characteristics of cells in tissue, for rat brains after blood removal by saline infusion at different infusion temperatures. To determine IOSs at each temperature, we first examined an isosbestic wavelength of the redox states of cytochtome c oxidase for each rat based on multiwavelength diffuse reflectance measurement. We then measured diffuse reflectance intensity at the isosbestic wavelength as a scattering signal, while diffuse reflectance intensity at 800 nm was detected to monitor the reduction of CuA in cytochrome c oxidase. At all temperatures, the scattering signal was steady in an early phase but showed a drastic, triphasic change in a certain time range of infusion; during this scattering change, the reduction of CuA started and proceeded rapidly. The start time of triphasic scattering change as well as the start time of the reduction of CuA was extended for more than 2 min by lowering infusion temperature from 30 to 23°C and we found that there was a linear correlation between these two start times. These results suggest that tissue metabolic activity can be maintained for longer time by keeping the brain at lower temperature, and triphasic scattering change can be used as an optical signal indicating the reduction of CuA in cytochrome c oxidase, and hence loss of tissue viability for brain.

Citation Download Citation

Satoko Kawauchi, Shunichi Sato, Hidetoshi Ooigawa, Hiroshi Nawashiro, and Makoto Kikuchi "Changes in intrinsic optical signals during loss of tissue viability of brains in rats: effect of brain temperature", Proc. SPIE 6434, Optical Tomography and Spectroscopy of Tissue VII, 64341O (26 February 2007); https://doi.org/10.1117/12.700209

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