Superresolution microscopy using common-path phase-shifting interferometry

Vicente Micó; Zeev Zalevsky; Javier García

doi:10.1117/12.780719

23 April 2008 Superresolution microscopy using common-path phase-shifting interferometry

Vicente Micó, Zeev Zalevsky, Javier García

Proceedings Volume 7000, Optical and Digital Image Processing; 700002 (2008) https://doi.org/10.1117/12.780719
Event: SPIE Photonics Europe, 2008, Strasbourg, France

Abstract

In this contribution, we present a new approach aimed to achieve superresolution in digital holographic microscopy that overcomes the limitations imposed by the Abbe's diffraction limit. Based on time multiplexing and off-axis illumination, a common-path interferometric architecture allows the synthesis of an enlarged aperture that expands the cutoff frequency of the conventional imaging system. Complex object amplitude distribution is recovered using an extremely simple phase-shifting configuration instead of image plane off-axis holographic recording. As a consequence, the setup becomes easy-to-configure (less requirements and lower number of optical elements) and it is useful for practical implementation in microscopes while only simple modifications are required (no higher magnifications are needed to resolve the interference pattern at the CCD plane). Experimental results validating the capabilities of the presented approach when a low numerical aperture commercial microscope objective is used as imaging lens are included using both a synthetic object (high resolution USAF test target) and a biosample (red blood cells) as objects under test.

Citation Download Citation

Vicente Micó, Zeev Zalevsky, and Javier García "Superresolution microscopy using common-path phase-shifting interferometry", Proc. SPIE 7000, Optical and Digital Image Processing, 700002 (23 April 2008); https://doi.org/10.1117/12.780719

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KEYWORDS

Diffraction gratings

Imaging systems

Microscopes

Holography

Phase shifts

Super resolution

Charge-coupled devices

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