Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Non-scanning CARS microscopy using wide-field geometry

[+] Author Affiliations
I. Toytman, K. Cohn, T. Smith, D. Simanovskii

Stanford Univ.

D. Palanker

Stanford Univ. and Stanford Univ. School of Medicine

Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 64420D (February 12, 2007); doi:10.1117/12.701617
Text Size: A A A
From Conference Volume 6442

  • Multiphoton Microscopy in the Biomedical Sciences VII
  • Ammasi Periasamy; Peter T. C. So
  • San Jose, CA | January 20, 2007

abstract

We report a wide-field Coherent Anti-Stokes Raman Scattering (CARS) microscopy technique based on non-phasematching illumination and imaging systems. This technique is based on a non-collinear sample illumination by broad laser beams and recording image of sample at anti-Stokes wavelength using full-frame image detector. An amplified Ti:Sapphire laser and an Optical Parametric Amplifier (OPA) provided picosecond pump and Stokes beams with energies sufficient for CARS generation in an area of 100 μm in diameter. The whole field of view of the microscope was illuminated simultaneously by the pump and Stokes beams, and CARS signal was recorded onto a cooled CCD, with resolution determined by the microscope objective. Several illumination schemes and several types of thin sample preparations have been explored. We demonstrated that CARS image of a 100x100 μm sample can be recorded with submicrometer spatial resolution using just a few laser pulses of microJoule energies.

© (2007) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

I. Toytman ; K. Cohn ; T. Smith ; D. Simanovskii and D. Palanker
"Non-scanning CARS microscopy using wide-field geometry", Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 64420D (February 12, 2007); doi:10.1117/12.701617; http://dx.doi.org/10.1117/12.701617


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.