Specifying tissue optical properties using axial dependence of confocal reflectance images: confocal scanning laser microscopy and optical coherence tomography

S. Jacques; R. Samatham; N. Choudhury; D. S. Gareau

doi:10.1117/12.716535

16 February 2007 Specifying tissue optical properties using axial dependence of confocal reflectance images: confocal scanning laser microscopy and optical coherence tomography

S. Jacques, R. Samatham, N. Choudhury, D. S. Gareau

Author Affiliations +

Proceedings Volume 6446, Biomedical Applications of Light Scattering; 64460N (2007) https://doi.org/10.1117/12.716535
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

The optical properties of a tissue can be specified by the depth dependence of a reflectance-mode confocal measurement, as the focus is scanned down into a tissue. Reflectance-mode confocal scanning laser microscopy (rCSLM) and optical coherence tomography in focus tracking mode (OCT) are two examples of such confocal measurements. The measurement of reflected signal as a function of the depth of focus, R(z), is expressed as ρe^-μz, where ρ [dimensionless] is the local reflectivity from the focus within a tissue and μ [cm^-1] is the attenuation of signal as a function of z. The reflectivity of a mirror defines ρ = 1. This paper describes how the experimental ρ and μ map into the optical properties of scattering coefficient, μs [cm^-1], and anisotropy of scattering, g [dimensionless]. Preliminary results on tissue for the rCSLM and OCT systems are reported.

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S. Jacques, R. Samatham, N. Choudhury, and D. S. Gareau "Specifying tissue optical properties using axial dependence of confocal reflectance images: confocal scanning laser microscopy and optical coherence tomography", Proc. SPIE 6446, Biomedical Applications of Light Scattering, 64460N (16 February 2007); https://doi.org/10.1117/12.716535

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