Simulation and modeling of optical phase contrast microscope cellular nanobioimaging

Stoyan Tanev; Valery V. Tuchin; James Pond

doi:10.1117/12.822518

19 December 2008 Simulation and modeling of optical phase contrast microscope cellular nanobioimaging

Stoyan Tanev, Valery V. Tuchin, James Pond

Proceedings Volume 7027, 15th International School on Quantum Electronics: Laser Physics and Applications; 702716 (2008) https://doi.org/10.1117/12.822518
Event: 15th International School on Quantum Electronics: Laser Physics and Applications, 2008, Bourgas, Bulgaria

Abstract

We apply a previously developed 3D Finite-Difference Time-Domain (FDTD) simulation method to model the optical phase contrast microscopic (OPCM) visualization of Gold nanoparticles (NPs) attached to the nucleus of a singe biological cell. We consider a realistic size 3D cell model at optical immersion conditions, i.e. when the refractive index values of the cytoplasm and of the extra-cellular medium are equal. The visual effect of the Gold NPs is studied at both resonant and non-resonant conditions. The results show that, at resonance, there are specific visual patterns that could be used for the identification of the presence of NPs at the nucleus' surface. The model demonstrates the capability to model the specific conditions of OPCM image enhancement by optically controlling the resonant properties of the NPs.

Citation Download Citation

Stoyan Tanev, Valery V. Tuchin, and James Pond "Simulation and modeling of optical phase contrast microscope cellular nanobioimaging", Proc. SPIE 7027, 15th International School on Quantum Electronics: Laser Physics and Applications, 702716 (19 December 2008); https://doi.org/10.1117/12.822518

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