Correction of Raman signals for tissue optical properties

Carina Reble; Ingo Gersonde; Jürgen Helfmann; Stefan Andree; Gerd Illing

doi:10.1117/12.831595

7 July 2009 Correction of Raman signals for tissue optical properties

Carina Reble, Ingo Gersonde, Jürgen Helfmann, Stefan Andree, Gerd Illing

Proceedings Volume 7368, Clinical and Biomedical Spectroscopy; 73680C (2009) https://doi.org/10.1117/12.831595
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

Raman signals of biological tissues are distorted by the influence of tissue absorption and scattering properties, which significantly challenges signal quantification. We investigated the influence of the tissue optical properties on the resonance Raman signal of β-carotene with tissue phantom measurements and Monte Carlo simulations. Both methods show that the Raman signal drops roughly proportional to 1/μ_a for absorption coefficients (μa) in the range of 0.1-4mm^-1. The influence of the reduced scattering coefficient in the range of 0.1-8mm^-1 is less strong, but not negligible. We introduce combined Raman and spatially resolved reflectance measurements to measure both Raman signals and tissue optical properties. The optical properties were deduced from the spatially resolved reflectance measurements by a Monte Carlo-based lookup table (LUT). For the signal correction we applied correction functions derived from the Monte Carlo simulations. We corrected in vivo resonance Raman measurements of carotenoids in caucasian skin (n=9) with respect to the mean optical properties of the group. The magnitude of the average correction effect was found to be 18±11% on the minimal pigmented palm.

Citation Download Citation

Carina Reble, Ingo Gersonde, Jürgen Helfmann, Stefan Andree, and Gerd Illing "Correction of Raman signals for tissue optical properties", Proc. SPIE 7368, Clinical and Biomedical Spectroscopy, 73680C (7 July 2009); https://doi.org/10.1117/12.831595

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