Interferometry for topographical diagnostics of RBCs in optical tweezers

Ranjeet Kumar; Sarita Saraswati; Chandra Shakher; Dalip S. Mehta

doi:10.1117/12.878959

24 November 2010 Interferometry for topographical diagnostics of RBCs in optical tweezers

Ranjeet Kumar, Sarita Saraswati, Chandra Shakher, Dalip S. Mehta

Proceedings Volume 7376, Laser Applications in Life Sciences; 73760G (2010) https://doi.org/10.1117/12.878959
Event: Laser Applications in Life Sciences 2010, 2010, Oulu, Finland

Abstract

Simultaneous non-invasive trapping and topography of erythrocytes by interferometric fringe projection profilometry has been presented. Sinusoidal fringes were generated with the help of compact Michelson interferometer (CMI) developed by coating a thin reflective layer (~100μm) of Al₂O₃ on one face of a cubic beam splitter. An external mirror was mounted on XYZ translational stage to control the fringe frequency and orientation. Red He-Ne laser was used to generate the sinusoidal probe -interferogram using CMI to project onto the green-laser (cw) trapped healthy and deceased RBCs separately. Information coded reflected interferograms exhibits characteristic fringe-deviation with respect to probe-pattern for both the healthy and defective RBCs. Fourier transform analysis was adopted to retrieve the phase-map which can be exploited for topography, size determination and refractive-index of RBC. Refractive-index change is directly related with hemoglobin concentration of RBCs at any specific physiological state and hence information about health status and disease progression can be anticipated.

Citation Download Citation

Ranjeet Kumar, Sarita Saraswati, Chandra Shakher, and Dalip S. Mehta "Interferometry for topographical diagnostics of RBCs in optical tweezers", Proc. SPIE 7376, Laser Applications in Life Sciences, 73760G (24 November 2010); https://doi.org/10.1117/12.878959

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KEYWORDS

Optical tweezers

Refractive index

Interferometry

Fourier transforms

Reflection

Beam splitters

Wavefronts

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