Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Measurement of light scattering from trapped particles

[+] Author Affiliations
Mikael Collins, Antti Kauppila, Matti Kinnunen, Risto Myllylä

Univ. of Oulu (Finland)

Artashes Karmenyan

National Yang-Ming Univ. (Taiwan)

Lech Gajewski, Krzysztof Szewczyk

Silesian Univ. of Technology in Gliwice (Poland)

Proc. SPIE 7376, Laser Applications in Life Sciences, 737619 (November 24, 2010); doi:10.1117/12.871613
Text Size: A A A
From Conference Volume 7376

  • Laser Applications in Life Sciences
  • Matti Kinnunen; Risto Myllylä
  • Oulu, Finland | June 09, 2010

abstract

We have developed an optical tweezers setup combined with a laser light scattering measurement system to measure the elastic light scattering from trapped particles. The setup consists of a near infrared laser (λ=1064nm), a water immersion objective for trapping, a single or double structure sample cuvette, and a HeNe-laser for illuminating the trapped particles. The light is detected with an amplified photomultiplier and a lock-in amplifier. Light scattering images from the trapped particles are also shown with a CCD camera. An optical trap keeps the particle stable during the measurement. The measured scattering patterns from 23.25 μm diameter polystyrene spheres were shown to have good comparability with theoretical modelling. 6.0 μm particles were also measured. The light scattering from trapped red blood cells was much weaker than that from 23.25 μm and 6.0 μm polystyrene spheres, almost at the detection limit of our current detection system configuration. The stability of the polystyrene sphere was much better during the measurements than that of the red blood cell.

© (2010) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Mikael Collins ; Antti Kauppila ; Artashes Karmenyan ; Lech Gajewski ; Krzysztof Szewczyk, et al.
"Measurement of light scattering from trapped particles", Proc. SPIE 7376, Laser Applications in Life Sciences, 737619 (November 24, 2010); doi:10.1117/12.871613; http://dx.doi.org/10.1117/12.871613


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.