Investigation of alveolar tissue deformations using OCT combined with fluorescence microscopy

Maria Gaertner; Peter Cimalla; Lilla Knels; Sven Meissner; Christian Schnabel; Wolfgang M. Kuebler; Edmund Koch

doi:10.1117/12.889942

1 June 2011 Investigation of alveolar tissue deformations using OCT combined with fluorescence microscopy

Maria Gaertner, Peter Cimalla, Lilla Knels, Sven Meissner, Christian Schnabel, Wolfgang M. Kuebler, Edmund Koch

Proceedings Volume 8091, Optical Coherence Tomography and Coherence Techniques V; 80911P (2011) https://doi.org/10.1117/12.889942
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

In critical care medicine, artificial ventilation is a life saving tool providing sufficient blood oxygenation to patients suffering from respiratory failure. Essential for their survival is the use of protective ventilation strategies to prevent further lung damage due to ventilator induced lung injury (VILI). Since there is only little known about implications of lung tissue overdistension on the alveolar level, especially in the case of diseased lungs, this research deals with the investigation of lung tissue deformation on a microscale. A combined setup utilizing optical coherence tomography (OCT) and confocal fluorescence microscopy, is used to study the elastic behavior of the alveolar tissue. Three-dimensional geometrical information with voxel sizes of 6 μm × 6 μm × 11 μm (in air) is provided by OCT, structural information about localization of elastin fibers is elucidated via confocal fluorescence microscopy with a lateral resolution of around 1 μm. Imaging depths of 90 μm for OCT and 20 μm for confocal fluorescence microscopy were obtained. Dynamic studies of subpleural tissue were carried out on the basis of an in vivo mouse model post mortem, mimicking the physiological environment of an intact thorax and facilitating a window for the application of optical methods. Morphological changes were recorded by applying constant positive airway pressures of different values. With this, alveolar volume changes could clearly be recognized and quantified to form a compliance value of 3.5 • 10^-6(see manuscript). The distribution of elastin fibers was detected and will be subject to further elasticity analysis.

Citation Download Citation

Maria Gaertner, Peter Cimalla, Lilla Knels, Sven Meissner, Christian Schnabel, Wolfgang M. Kuebler, and Edmund Koch "Investigation of alveolar tissue deformations using OCT combined with fluorescence microscopy", Proc. SPIE 8091, Optical Coherence Tomography and Coherence Techniques V, 80911P (1 June 2011); https://doi.org/10.1117/12.889942

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available