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Proceedings Article

Dark-field optical coherence microscopy

[+] Author Affiliations
C. Pache, M. L. Villiger, T. Lasser

Ecole Polytechnique Fédérale de Lausanne (Switzerland)

Proc. SPIE 7554, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV, 755425 (February 19, 2010); doi:10.1117/12.842848
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From Conference Volume 7554

  • Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV
  • Joseph A. Izatt; James G. Fujimoto; Valery V. Tuchin
  • San Francisco, California | January 23, 2010

abstract

Many solutions have been proposed to produce phase quantitative images of biological cell samples. Among these, Spectral Domain Phase Microscopy combines the fast imaging speed and high sensitivity of Optical Coherence Microscopy (OCM) in the Fourier domain with the high phase stability of common-path interferometry. We report on a new illumination scheme for OCM that enhances the sensitivity for backscattered light and detects the weak sample signal, otherwise buried by the signal from specular reflection. With the use of a Bessel-like beam, a dark-field configuration was realized. Sensitivity measurements for three different illumination configurations were performed to compare our method to standard OCM and extended focus OCM. Using a well-defined scattering and reflecting object, we demonstrated an attenuation of -40 dB of the DC-component and a relative gain of 30 dB for scattered light, compared to standard OCM. In a second step, we applied this technique, referred to as dark-field Optical Coherence Microscopy (dfOCM), to living cells. Chinese hamster ovarian cells were applied in a drop of medium on a coverslide. The cells of ~15 μm in diameter and even internal cell structures were visualized in the acquired tomograms.

© (2010) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

C. Pache ; M. L. Villiger and T. Lasser
"Dark-field optical coherence microscopy", Proc. SPIE 7554, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV, 755425 (February 19, 2010); doi:10.1117/12.842848; http://dx.doi.org/10.1117/12.842848


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