Photoacoustic microscopy with submicron resolution

Konstantin Maslov; Geng Ku; Lihong V. Wang

doi:10.1117/12.841919

23 February 2010 Photoacoustic microscopy with submicron resolution

Konstantin Maslov, Geng Ku, Lihong V. Wang

Proceedings Volume 7564, Photons Plus Ultrasound: Imaging and Sensing 2010; 75640W (2010) https://doi.org/10.1117/12.841919
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We show that it is possible to obtain high optical contrast photoacoustic images of tissue with 0.55 μm transverse resolution. To achieve high sensitivity, we used a high NA (0.85), 125 MHz spherically focused ultrasonic transducer in a confocal arrangement with a high resolution optical objective (NA=0.6). Laser pulses of a few nJ in pulse energy with durations of 1.5 ns at a 20 KHz pulse repetition rate were used to generate photoacoustic waves. Although the penetration depth is limited to hundreds of microns by both optical scattering and ultrasonic absorption, the developed technique can compete with optical microscopy, for example, in quantitative spectral measurements, in microcirculation research, or in nanoparticle detection.

Citation Download Citation

Konstantin Maslov, Geng Ku, and Lihong V. Wang "Photoacoustic microscopy with submicron resolution", Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75640W (23 February 2010); https://doi.org/10.1117/12.841919

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