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Proceedings Article

Optical-resolution photoacoustic microscopy of amyloid-β deposits in vivo

[+] Author Affiliations
Song Hu, Ping Yan, Konstantin Maslov, Jin-Moo Lee, Lihong V. Wang

Washington Univ. in St. Louis (USA)

Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75643D (February 23, 2010); doi:10.1117/12.843919
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From Conference Volume 7564

  • Photons Plus Ultrasound: Imaging and Sensing 2010
  • Alexander A. Oraevsky; Lihong V. Wang
  • San Francisco, California | January 23, 2010

abstract

Advances in high-resolution imaging have permitted microscopic observations within the brains of living animals. Applied to Alzheimer's disease (AD) mouse models, multiphoton microscopy has opened a new window to study the real-time appearance and growth of amyloid plaques. Here, we report an alternative technology-optical-resolution photoacoustic microscopy (OR-PAM)-for in vivo imaging of amyloid plaques in a transgenic AD mouse model. In vivo validation using multiphoton microscopy shows that OR-PAM has sufficient sensitivity and spatial resolution to identify amyloid plaques in living brains. In addition, with dual-wavelength OR-PAM, the three-dimensional morphology of amyloid plaques and the surrounding microvasculature are imaged simultaneously through a cranial window. In vivo transcranial OR-PAM imaging of amyloid plaques is highly likely once the imaging parameters are optimized.

© (2010) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Song Hu ; Ping Yan ; Konstantin Maslov ; Jin-Moo Lee and Lihong V. Wang
"Optical-resolution photoacoustic microscopy of amyloid-β deposits in vivo", Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75643D (February 23, 2010); doi:10.1117/12.843919; http://dx.doi.org/10.1117/12.843919


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