Reflectometric interference biosensing using nanopores: integration into microfluidics

Tushar Kumeria; Mahaveer Kurkuri; Kerrilyn Diener; Chen Zhang; Luke Parkinson; Dusan Losic

doi:10.1117/12.903217

23 December 2011 Reflectometric interference biosensing using nanopores: integration into microfluidics

Tushar Kumeria, Mahaveer Kurkuri, Kerrilyn Diener, Chen Zhang, Luke Parkinson, Dusan Losic

Proceedings Volume 8204, Smart Nano-Micro Materials and Devices; 82043C (2011) https://doi.org/10.1117/12.903217
Event: SPIE Smart Nano + Micro Materials and Devices, 2011, Melbourne, Australia

Abstract

The concept of a microfluidic biosensing device based on reflective interferometric spectroscopy (RIfS) is presented in this article. The key element of the sensor is a highly ordered nanoporous structure of anodic aluminium oxide (AAO) integrated into a microfluidic chip combined with an optical fiber spectrophotometer. AAO was prepared by electrochemical anodization of aluminium using 0.3 M oxalic acid. The structural and geometrical features of the AAO porous structures were controlled to provide optimal RIfS sensing characteristics and there sensing capabilities were explored using two different strategies; i) detection based on the response generated by pefusion of analyte ions inside the pores and ii) detection based on specific adsorption of analyte molecules on surface of AAO pores. The second strategy is based on chemical modification of the AAO surface to target molecules based on specific surface binding reactions. In this work two cases are presented, including the binding of small thiol molecules on gold-modified AAO (Au-AAO) and binding of larger targets such as circulating tumour cells (CTC) on antibody-modified AAO. Our preliminary results show an excellent capability of our system in the detection of different analytes using both strategies, and confirm good potential for the development and application of interferometric label-free biosensing devices in a wide range of biomedical applications.

Citation Download Citation

Tushar Kumeria, Mahaveer Kurkuri, Kerrilyn Diener, Chen Zhang, Luke Parkinson, and Dusan Losic "Reflectometric interference biosensing using nanopores: integration into microfluidics", Proc. SPIE 8204, Smart Nano-Micro Materials and Devices, 82043C (23 December 2011); https://doi.org/10.1117/12.903217

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KEYWORDS

Sensors

Microfluidics

Molecules

Aluminum

Biosensing

Gold

Refractive index

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