Multi-functional stage-scanning fluorescence micro/nanoscope for single-lipid dynamics

Li-Ling Yang; Chia-Fen Hsieh; Yi-Ren Chang; Jie-Pan Shen; Yu-Chung Chang; Chia-Fu Chou

doi:10.1117/12.908179

13 February 2012 Multi-functional stage-scanning fluorescence micro/nanoscope for single-lipid dynamics

Li-Ling Yang, Chia-Fen Hsieh, Yi-Ren Chang, Jie-Pan Shen, Yu-Chung Chang, Chia-Fu Chou

Proceedings Volume 8228, Single Molecule Spectroscopy and Superresolution Imaging V; 822804 (2012) https://doi.org/10.1117/12.908179
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

We combine pulsed laser, supercontinuum radiation source and fast single-photon counting peripherals to obtain a multifunctional micro/nano-scope. This provides us with better spatial and temporal resolution to observe fast dynamics. Performing fluorescence correlation spectroscopy for fast dynamics (<μs) with sub-diffraction limit resolution to observe the initial state of single-lipid dynamics in supported lipid bilayers and living cells is our goal. Lipid raft serves as a platform for recruiting signaling components of effective signal transduction. However, the dynamics of sub-200nm rapidly aggregated lipid rafts are still not elucidated in living cells. We here report our recent progress on the construction of this multi-functional stage-scanning fluorescence micro/nanoscope for single-lipid dynamics study.

Citation Download Citation

Li-Ling Yang, Chia-Fen Hsieh, Yi-Ren Chang, Jie-Pan Shen, Yu-Chung Chang, and Chia-Fu Chou "Multi-functional stage-scanning fluorescence micro/nanoscope for single-lipid dynamics", Proc. SPIE 8228, Single Molecule Spectroscopy and Superresolution Imaging V, 822804 (13 February 2012); https://doi.org/10.1117/12.908179

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