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Proceedings Article

High resolution surface plasmon microscopy for cell imaging

[+] Author Affiliations
F. Argoul, T. Roland, L. Berguiga

Lab. Joliot-Curie and Lab. de Physique, CNRS, Ecole Normale Supérieure de Lyon (France)

K. Monier

Lab. Joliot-Curie, CNRS, Ecole Normale Supérieure de Lyon (France)

J. Elezgaray

Lab. de Microbiologie et Biochimie Appliquée, CNRS, Univ. Bordeaux 1 (France)

Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 771506 (May 14, 2010); doi:10.1117/12.853856
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From Conference Volume 7715

  • Biophotonics: Photonic Solutions for Better Health Care II
  • Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews
  • Brussels, Belgium | April 12, 2010

abstract

We introduce a new non-labeling high resolution microscopy method for cellular imaging. This method called SSPM (Scanning Surface Plasmon Microscopy) pushes down the resolution limit of surface plasmon resonance imaging (SPRi) to sub-micronic scales. High resolution SPRi is obtained by the surface plasmon lauching with a high numerical aperture objective lens. The advantages of SPPM compared to other high resolution SPRi's rely on three aspects; (i) the interferometric detection of the back reflected light after plasmon excitation, (ii) the twodimensional scanning of the sample for image reconstruction, (iii) the radial polarization of light, enhancing both resolution and sensitivity. This microscope can afford a lateral resolution of - 150 nm in liquid environment and - 200 nm in air. We present in this paper images of IMR90 fibroblasts obtained with SSPM in dried environment. Internal compartments such as nucleus, nucleolus, mitochondria, cellular and nuclear membrane can be recognized without labelling. We propose an interpretation of the ability of SSPM to reveal high index contrast zones by a local decomposition of the V (Z) function describing the response of the SSPM.

© (2010) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

F. Argoul ; K. Monier ; T. Roland ; J. Elezgaray and L. Berguiga
"High resolution surface plasmon microscopy for cell imaging", Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 771506 (May 14, 2010); doi:10.1117/12.853856; http://dx.doi.org/10.1117/12.853856


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