Fiber-optic confocal microscopy using a miniaturized needle-compatible imaging probe

Rajesh S. Pillai; Dirk Lorenser; David D. Sampson

doi:10.1117/12.887373

17 May 2011 Fiber-optic confocal microscopy using a miniaturized needle-compatible imaging probe

Rajesh S. Pillai, Dirk Lorenser, David D. Sampson

Proceedings Volume 7753, 21st International Conference on Optical Fiber Sensors; 77534M (2011) https://doi.org/10.1117/12.887373
Event: 21st International Conference on Optical Fibre Sensors (OFS21), 2011, Ottawa, Canada

Abstract

We report on the design and implementation of a 350 μm-diameter confocal imaging probe based on gradient-index (GRIN) optics and a fiber-based scanning arrangement. The form factor of the probe is such that it can potentially be inserted into a 22-gauge hypodermic needle to perform high-resolution confocal fluorescence imaging in solid tissues. We introduce a simple scanning arrangement based on lensed fiber, which eliminates off-axis aberrations induced by conventional scanning optics and is suitable for integration into a compact hand-held unit. We present the details of the optical design and experimental verification of the performance of the optical system. The measured lateral resolution of ~700 nm is in agreement with the optical design and is the highest resolution reported for a confocal fluorescence imaging probe of this size. Further, we demonstrate the imaging capability of the probe by obtaining high-resolution images of fluorescently labeled muscle fibers.

Citation Download Citation

Rajesh S. Pillai, Dirk Lorenser, and David D. Sampson "Fiber-optic confocal microscopy using a miniaturized needle-compatible imaging probe", Proc. SPIE 7753, 21st International Conference on Optical Fiber Sensors, 77534M (17 May 2011); https://doi.org/10.1117/12.887373

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