Using wide-field quantitative diffuse reflectance spectroscopy in combination with high-resolution imaging for margin assessment

Stephanie Kennedy; Jenna Mueller; Torre Bydlon; J. Quincy Brown; Nimmi Ramanujam

doi:10.1117/12.875735

23 February 2011 Using wide-field quantitative diffuse reflectance spectroscopy in combination with high-resolution imaging for margin assessment

Stephanie Kennedy, Jenna Mueller, Torre Bydlon, J. Quincy Brown, Nimmi Ramanujam

Author Affiliations +

Proceedings Volume 7890, Advanced Biomedical and Clinical Diagnostic Systems IX; 78900C (2011) https://doi.org/10.1117/12.875735
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Due to the large number of women diagnosed with breast cancer and the lack of intra-operative tools, breast cancer margin assessment presents a significant unmet clinical need. Diffuse reflectance spectral imaging provides a method for quantitatively interrogating margins of lumpectomy specimens. We have previously found that [β- carotene]/μ_s' is a diagnostically important parameter but both parameters, [β-carotene] and μ_s', were derived from a low resolution parameter map and are subject to the tissue type and heterogeneity present in the breast. In this study, we used diffuse reflectance measurements from individual sites co-registered with high resolution microendoscopy (HRME) images to determine if the combined performance of these technologies could improve margin assessment. By comparing the optical parameters of [β-carotene] and μ_s' to the quantitative HRME image endpoints of feature size, feature density and normalized fluorescence, we determined that adding HRME to spectral imaging can improve the specificity of our diffuse reflectance spectral imaging system.

Citation Download Citation

Stephanie Kennedy, Jenna Mueller, Torre Bydlon, J. Quincy Brown, and Nimmi Ramanujam "Using wide-field quantitative diffuse reflectance spectroscopy in combination with high-resolution imaging for margin assessment", Proc. SPIE 7890, Advanced Biomedical and Clinical Diagnostic Systems IX, 78900C (23 February 2011); https://doi.org/10.1117/12.875735

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