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Proceedings Article

Fluorescence lifetime imaging endoscopy

[+] Author Affiliations
G. T. Kennedy, S. Coda, A. J. Thompson, D. S. Elson, M. A. A. Neil, G. W. Stamp, A. Thillainayagam, C. Dunsby, Paul M. W. French

Imperial College London (United Kingdom)

B. Viellerobe, F. Lacombe

Mauna Kea Technologies (France)

Proc. SPIE 7893, Endoscopic Microscopy VI, 789308 (February 22, 2011); doi:10.1117/12.874968
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From Conference Volume 7893

  • Endoscopic Microscopy VI
  • Guillermo J. Tearney; Thomas D. Wang
  • San Francisco, California, USA | January 22, 2011


We present two FLIM endoscopes for clinical imaging and in vivo cell biology. For subcellular confocal imaging we demonstrated the first confocal FLIM endomicroscope, implementing TCSPC with a Cellvizio®GI, which we have now developed as a self-contained wheeled instrument (1.0 × 0.7 m) incorporating a tunable excitation laser and acquiring images in < 10 s. This has been applied to image FRET in live cells and to image tissue autofluorescence, for which we are implementing "FIFO" for image montaging. For diagnostic screening/guided biopsy, we have developed a complementary wide-field FLIM endoscope employing time-gated detection with violet and UV excitation for imaging over mm-cm fields of view.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.

G. T. Kennedy ; S. Coda ; A. J. Thompson ; D. S. Elson ; M. A. A. Neil, et al.
"Fluorescence lifetime imaging endoscopy", Proc. SPIE 7893, Endoscopic Microscopy VI, 789308 (February 22, 2011); doi:10.1117/12.874968; http://dx.doi.org/10.1117/12.874968

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