Fluorescence lifetime imaging endoscopy

G. T. Kennedy; S. Coda; A. J. Thompson; D. S. Elson; M. A. A. Neil; G. W Stamp; A. Thillainayagam; B. Viellerobe; F. Lacombe; C. Dunsby; Paul M. W. French

doi:10.1117/12.874968

22 February 2011 Fluorescence lifetime imaging endoscopy

G. T. Kennedy, S. Coda, A. J. Thompson, D. S. Elson, M. A. A. Neil, G. W Stamp, A. Thillainayagam, B. Viellerobe, F. Lacombe, C. Dunsby, Paul M. W. French

Author Affiliations +

Proceedings Volume 7893, Endoscopic Microscopy VI; 789308 (2011) https://doi.org/10.1117/12.874968
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

We present two FLIM endoscopes for clinical imaging and in vivo cell biology. For subcellular confocal imaging we demonstrated the first confocal FLIM endomicroscope, implementing TCSPC with a Cellvizio®GI, which we have now developed as a self-contained wheeled instrument (1.0 × 0.7 m) incorporating a tunable excitation laser and acquiring images in < 10 s. This has been applied to image FRET in live cells and to image tissue autofluorescence, for which we are implementing "FIFO" for image montaging. For diagnostic screening/guided biopsy, we have developed a complementary wide-field FLIM endoscope employing time-gated detection with violet and UV excitation for imaging over mm-cm fields of view.

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G. T. Kennedy, S. Coda, A. J. Thompson, D. S. Elson, M. A. A. Neil, G. W Stamp, A. Thillainayagam, B. Viellerobe, F. Lacombe, C. Dunsby, and Paul M. W. French "Fluorescence lifetime imaging endoscopy", Proc. SPIE 7893, Endoscopic Microscopy VI, 789308 (22 February 2011); https://doi.org/10.1117/12.874968

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