Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Fluorescence lifetime imaging endoscopy

[+] Author Affiliations
G. T. Kennedy, S. Coda, A. J. Thompson, D. S. Elson, M. A. A. Neil, G. W. Stamp, A. Thillainayagam, C. Dunsby, Paul M. W. French

Imperial College London (United Kingdom)

B. Viellerobe, F. Lacombe

Mauna Kea Technologies (France)

Proc. SPIE 7893, Endoscopic Microscopy VI, 789308 (February 22, 2011); doi:10.1117/12.874968
Text Size: A A A
From Conference Volume 7893

  • Endoscopic Microscopy VI
  • Guillermo J. Tearney; Thomas D. Wang
  • San Francisco, California, USA | January 22, 2011

abstract

We present two FLIM endoscopes for clinical imaging and in vivo cell biology. For subcellular confocal imaging we demonstrated the first confocal FLIM endomicroscope, implementing TCSPC with a Cellvizio®GI, which we have now developed as a self-contained wheeled instrument (1.0 × 0.7 m) incorporating a tunable excitation laser and acquiring images in < 10 s. This has been applied to image FRET in live cells and to image tissue autofluorescence, for which we are implementing "FIFO" for image montaging. For diagnostic screening/guided biopsy, we have developed a complementary wide-field FLIM endoscope employing time-gated detection with violet and UV excitation for imaging over mm-cm fields of view.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

G. T. Kennedy ; S. Coda ; A. J. Thompson ; D. S. Elson ; M. A. A. Neil, et al.
"Fluorescence lifetime imaging endoscopy", Proc. SPIE 7893, Endoscopic Microscopy VI, 789308 (February 22, 2011); doi:10.1117/12.874968; http://dx.doi.org/10.1117/12.874968


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.