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Proceedings Article

Taxol-induced paraptosis-like A549 cell death is not senescence

[+] Author Affiliations
Chao-yang Wang, Tong-Sheng Chen

South China Normal Univ. (China)

Proc. SPIE 7900, Biophotonics and Immune Responses VI, 79000K (February 10, 2011); doi:10.1117/12.877056
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From Conference Volume 7900

  • Biophotonics and Immune Responses VI
  • Wei R. Chen
  • San Francisco, California, USA | January 22, 2011

abstract

Our previous studies have shown that taxol, a potent anticancer agent, induces caspase-independent cell death and cytoplasmic vacuolization in human lung cancer cells. However, the mechanisms of taxol-induced cytoplasmic vacuolization are poorly understood. Cytoplasmic vacuolization have been reported to be a characteristic of cell senescence. Here, we employed confocal fluorescence microscopy imaging to study the reversibility of taxol-induced cytoplasmic vacuolization and whether taxol triggers senescence in A549 cells. We found that taxol-induced cytoplasmic vacuolization at 6 or 9 h after treatment with taxol did not decrease but increase at 24 h or 72 h after refreshing the culture medium without taxol, indicating taxol-induced cytoplasmic vacuolization is irreversible. We used SA-β-Gal (senescence-associated β-galactosidase) to assess whether taxol-induced cell death in cytoplasmic vacuolization fashion is senescence, and found that hydrogen peroxide (H2O2)-treated, but not taxol-treated cells is significantly stained by the SA-β-Gal, a senescence testing kit, indicating that the form of taxol-induced cell death is not senescence.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Chao-yang Wang and Tong-Sheng Chen
"Taxol-induced paraptosis-like A549 cell death is not senescence", Proc. SPIE 7900, Biophotonics and Immune Responses VI, 79000K (February 10, 2011); doi:10.1117/12.877056; http://dx.doi.org/10.1117/12.877056


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