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Proceedings Article

Development of a stigmatic imaging mass spectrometer using laser desorption/ionization

[+] Author Affiliations
Kunio Awazu

Osaka Univ. (Japan) and Univ. of Fukui (Japan) and Kyoto Univ. (Japan) and Japan Science and Technology Agency (Japan)

Hisanao Hazama, Hirofumi Nagao, Hidetoshi Yoshimura, Jun Aoki, Michisato Toyoda

Osaka Univ. (Japan) and Japan Science and Technology Agency (Japan)

Kenichi Fujii

Osaka Institute of Technology (Japan) and Japan Science and Technology Agency (Japan)

Katsuyoshi Masuda

Suntory Institute for Bioorganic Research (Japan) and Japan Science and Technology Agency (Japan)

Toshio Tashima

Japan Science and Technology Agency (Japan)

Yasuhide Naito

Graduate School for the Creation of New Photonics Industries (Japan) and Japan Science and Technology Agency (Japan)

Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 790225 (February 22, 2011); doi:10.1117/12.876005
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From Conference Volume 7902

  • Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
  • Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif
  • San Francisco, California, USA | January 22, 2011

abstract

Imaging mass spectrometry based on matrix-assisted laser desorption/ionization gives distributions of multiple biomolecules and identification of analytes in a tissue section. We are developing a stigmatic imaging mass spectrometer using a multi-turn time-of-flight mass spectrometer, MULTUM-IMG. A mesh pattern image was obtained with spatial resolution of 3 μm. Separation of stigmatic ion images according to the mass-to-charge ratio was also successfully demonstrated with the micro-dot pattern made with crystal violet and methylene blue. An ion image of a micro-dot pattern sample was observed after ten cycles in the multi-turn circuit of MULTUM-IMG, and the pattern of the sample was maintained. Then, stigmatic ion images of the ions of crystal violet and methylene blue produced from the stained section of a brain were observed. An ion image of whole part of a hippocampus (3.25 mm × 1.5 mm) in the brain section can be obtained within 13 minutes.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Kunio Awazu ; Hisanao Hazama ; Hirofumi Nagao ; Hidetoshi Yoshimura ; Jun Aoki, et al.
"Development of a stigmatic imaging mass spectrometer using laser desorption/ionization", Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 790225 (February 22, 2011); doi:10.1117/12.876005; http://dx.doi.org/10.1117/12.876005


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