Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Three-dimensional polarization second harmonic generation (3D-PSHG) imaging: the effect of the tilted-off the plane SHG active structures

[+] Author Affiliations
Sotiris Psilodimitrakopoulos, Ivan Amat-Roldan, Pablo Loza-Alvarez

ICFO - Instituto de Cièncias Fotònicas (Spain)

David Artigas

ICFO - Instituto de Cièncias Fotònicas (Spain) and Univ. Politècnica de Catalunya (Spain)

Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79030H (February 10, 2011); doi:10.1117/12.875254
Text Size: A A A
From Conference Volume 7903

  • Multiphoton Microscopy in the Biomedical Sciences XI
  • Ammasi Periasamy; Karsten König; Peter T. C. So
  • San Francisco, California, USA | January 22, 2011

abstract

Polarization sensitive second harmonic generation (PSHG) provides additional information in intensity only SHG imaging. In particular, it offers the SHG effective orientation of the implicated SHG active structures. Assuming that those structures possess cylindrical symmetry, the supplementary contrast is based on the ratio of two non-vanishing, independent elements of the χ2 tensor. This ratio is experimentally extracted by fitting pixel by pixel a theoretical model to the PSHG images and by finding the maximum frequency value or the mean of the consequent pixels' histogram. In the present study we show that the above χ2 elements' ratio critically depends on the tilted-off the plane SHG active structures. We performed PSHG in different z-planes of a starch granule (presenting radially oriented amylopectin molecules, the SHG source in starch) and we found different pick values of the χ2 elements' ratio histogram for each plane. By assuming a fixed value for the χ2 elements' ratio, we present here a generalized three dimensional (3D) model that determines the 3D orientation of the SHG active structures.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Sotiris Psilodimitrakopoulos ; Ivan Amat-Roldan ; David Artigas and Pablo Loza-Alvarez
"Three-dimensional polarization second harmonic generation (3D-PSHG) imaging: the effect of the tilted-off the plane SHG active structures", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79030H (February 10, 2011); doi:10.1117/12.875254; http://dx.doi.org/10.1117/12.875254


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.