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Proceedings Article

High contrast coherent anti-Stokes Raman scattering microscopy using tightly focused cylindrical vector beams

[+] Author Affiliations
Jian Lin, Fake Lu, Wei Zheng, Zhiwei Huang

National Univ. of Singapore (Singapore)

Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031F (February 22, 2011); doi:10.1117/12.873674
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From Conference Volume 7903

  • Multiphoton Microscopy in the Biomedical Sciences XI
  • Ammasi Periasamy; Karsten König; Peter T. C. So
  • San Francisco, California, USA | January 22, 2011

abstract

We report on a unique annular aperture detection scheme in radially and linearly polarized coherent anti-Stokes Raman scattering (RP- and LP-CARS) microscopy to effectively remove the solvent background for high contrast vibrational imaging. Our finite-difference time-domain (FDTD) calculations show that the far-field CARS radiation from the scatterer with size comparable to the excitation wavelength is stronger than that from the solvent at large cone angles (from 45° to the maximum collection angle of the objective). The annular detection provides about one to two orders higher contrast for forward detected RP- and LP-CARS microscopy.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Jian Lin ; Fake Lu ; Wei Zheng and Zhiwei Huang
"High contrast coherent anti-Stokes Raman scattering microscopy using tightly focused cylindrical vector beams", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031F (February 22, 2011); doi:10.1117/12.873674; http://dx.doi.org/10.1117/12.873674


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