Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Time-resolved fluorescence microscopy to study biologically related applications using sol-gel derived and cellular media

[+] Author Affiliations
Marion Toury, Archie Allison, David Campbell, David McLoskey, Graham Hungerford

HORIBA Jobin Yvon IBH Ltd. (United Kingdom)

Lin Chandler

HORIBA Scientific, Inc. (USA)

A. Sheila Holmes-Smith

Glasgow Caledonian Univ. (United Kingdom)

Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031W (February 22, 2011); doi:10.1117/12.882088
Text Size: A A A
From Conference Volume 7903

  • Multiphoton Microscopy in the Biomedical Sciences XI
  • Ammasi Periasamy; Karsten König; Peter T. C. So
  • San Francisco, California, USA | January 22, 2011

abstract

Fluorescence microscopy provides a non-invasive means for visualising dynamic protein interactions. As well as allowing the calculation of kinetic processes via the use of time-resolved fluorescence, localisation of the protein within cells or model systems can be monitored. These fluorescence lifetime images (FLIM) have become the preferred technique for elucidating protein dynamics due to the fact that the fluorescence lifetime is an absolute measure, in the main independent of fluorophore concentration and intensity fluctuations caused by factors such as photobleaching. In this work we demonstrate the use of a time-resolved fluorescence microscopy, employing a high repetition rate laser excitation source applied to study the influence of a metal surface on fluorescence tagged protein and to elucidate viscosity using the fluorescence lifetime probe DASPMI. These were studied in a cellular environment (yeast) and in a model system based on a sol-gel derived material, in which silver nanostructures were formed in situ using irradiation from a semiconductor laser in CW mode incorporated on a compact time-resolved fluorescence microscope (HORIBA Scientific DeltaDiode and DynaMyc).

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Marion Toury ; Lin Chandler ; Archie Allison ; David Campbell ; David McLoskey, et al.
"Time-resolved fluorescence microscopy to study biologically related applications using sol-gel derived and cellular media", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031W (February 22, 2011); doi:10.1117/12.882088; http://dx.doi.org/10.1117/12.882088


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.