Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

A femtosecond stimulated Raman loss (fSRL) microscope for highly sensitive bond-selective imaging

[+] Author Affiliations
Delong Zhang, Mikhail N. Slipchenko, Shuhua Yue, Junjie Li, Ji-Xin Cheng

Purdue Univ. (USA)

Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032L (February 28, 2011); doi:10.1117/12.875886
Text Size: A A A
From Conference Volume 7903

  • Multiphoton Microscopy in the Biomedical Sciences XI
  • Ammasi Periasamy; Karsten König; Peter T. C. So
  • San Francisco, California, USA | January 22, 2011

abstract

We demonstrate nonlinear vibrational imaging of isolated Raman bands by detecting femtosecond pulse stimulated Raman loss. Femtosecond pulse excitation produces a stimulated Raman loss signal that is 12 times larger than what picosecond pulse excitation produces. The strong signal allowed real-time, bond-selective imaging of deuterated palmitic acid-d31 inside live cells, and 3D sectioning of fat storage in live C. elegans. With the high peak power provided by femtosecond pulses, this system is highly compatible with other nonlinear optical modalities such as two-photon excited fluorescence. With most of the excitation power contributed by the Stokes beam in the 1.0 - 1.2 μm wavelength range, photodamage of biological samples was not observed.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Delong Zhang ; Mikhail N. Slipchenko ; Shuhua Yue ; Junjie Li and Ji-Xin Cheng
"A femtosecond stimulated Raman loss (fSRL) microscope for highly sensitive bond-selective imaging", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032L (February 28, 2011); doi:10.1117/12.875886; http://dx.doi.org/10.1117/12.875886


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.