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Proceedings Article

Visualization of heat propagation in biological tissues with two-photon fluorescence microscopy

[+] Author Affiliations
Chih-Yuan Yang, Chien-Sheng Liao, Yu-Yi Tzeng, Shi-Wei Chu

National Taiwan Univ. (Taiwan)

Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 790338 (February 10, 2011); doi:10.1117/12.875026
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From Conference Volume 7903

  • Multiphoton Microscopy in the Biomedical Sciences XI
  • Ammasi Periasamy; Karsten König; Peter T. C. So
  • San Francisco, California, USA | January 22, 2011

abstract

In this study, we have demonstrated by using time-lapsed two-photon fluorescence microscopy (2PFM), heat flow in biological tissues can be monitored based on temperature-induced fluorescence change of endogenous molecules. Compared to conventional infrared thermography or other thermal imaging tools, 2PFM provides not only sub-micrometer spatial resolution, but also optical sectioning capability inside tissues. As a proof of principle, 2PFM was used to monitor the fluorescence variation in a leaf of Eucalyptus Robusta Smith , which is placed on a home-made heater. By heating the leaf asymmetrically, significant changes of chlorophyll fluorescence were observed in the temperature range of 30°C to 60°C, leading to the visualization of heat conduction dynamics. From the analysis of temporal variation in fluorescence, 0.5°C thermal resolution was demonstrated and detailed temperature distribution can be quantized. Such a technique will be useful for the characterization of heat-related biophysical mechanisms with high spatial and temperature resolution in a thick tissue.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Chih-Yuan Yang ; Chien-Sheng Liao ; Yu-Yi Tzeng and Shi-Wei Chu
"Visualization of heat propagation in biological tissues with two-photon fluorescence microscopy", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 790338 (February 10, 2011); doi:10.1117/12.875026; http://dx.doi.org/10.1117/12.875026


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